Publications
2026
Compartment-specific collagen architecture in Crohn’s disease biopsies using SHG/TPE microscopy: a pilot feasibility study
Tariq, Raseen, et al., Journal of Histotechnology (2026): 1-5.
Steatosis in Cirrhosis: A Prognostic Marker for Liver-Related Outcomes in Metabolic-Dysfunction Associated Steatotic Liver Disease
Pei, Yiying, et al., JOURNAL OF HEPATOLOGY. Vol. 82. RADARWEG 29, 1043 NX AMSTERDAM, NETHERLANDS: ELSEVIER, 2025.
AI digital pathology as a key tool providing in-depth understanding of the progression and regression of MASH and fibrosis in male mouse models
Saigal, Ashmita, et al., Nature Communications 17.1 (2026): 6964.
Real-World Insights in Designing SteatoStat: An End-to-End Deep Learning Pipeline for Hepatic Steatosis Quantification
Jegannathan, Nagalakshmi, et al., Diagnostics (2026).
The Asian Pacific Association of the Study of the Liver expert survey on artificial intelligence‐assisted reporting of liver histopathology in metabolic dysfunction associated fatty liver disease
Elangovan, H., et al., Hepatology International (2026): 1-11.
Quantitative regression of qFibrosis with resmetirom: Exploratory histologic endpoints from the MAESTRO-NASH phase III clinical trial
Schattenberg, Jörn M., et al., Journal of Hepatology (2026)
Multicenter Prospective Cohort Study on Clinical Outcomes and Fibrosis Patterns in Biopsy-Proven Steatotic Liver Disease Subtypes
Kim, Gi-Ae, et al., Gastroenterology (2026).
AI-assisted fibrosis scoring in MASH: Exploring pathologist decision-making with an SHG-based AI digital pathology tool
Abdurrachim, Desiree, et al. JHEP Reports (2026): 101752.
2025
The Relationship Between Non-Invasive Tests and Digital Pathology for Quantifying Liver Fibrosis in MASLD
Wei, Xiaodie, et al. Diagnostics 15.19 (2025): 2475.
Development of AI Based Fibrosis Detection Algorithm by SHG/TPEF Microscopy for Fully Quantified Liver Fibrosis Assessment in MASH
Akbary, Kutbuddin, et al. Liver International 45.9 (2025): e70258.
Repeatability and reproducibility of artificial imaging based digital pathology for the evaluation of liver fibrosis
Ren, Yayun et al., Clinical Gastroenterology and Hepatology (2025)
Quantitative fibrosis identifies biliary tract involvement and is associated with outcomes in pediatric autoimmune liver disease
Khendek, Leticia, et al., Hepatology Communications 9.1 (2025): e0594.
Effect of liver biopsy size on MASLD fibrosis assessment by second-harmonic generation/two-photon excitation fluorescence microscopy
Field, Daniel T., et al., JHEP Reports (2025): 101449.
The Emerging Role of Second Harmonic Generation/Two Photon Excitation for Precision Digital Analysis of Liver Fibrosis in MASH Clinical Trials
Neuschwander-Tetri, Brent A., et al., Journal of Hepatology (2025). This study has been featured on the cover of the Journal of Hepatology.
Second harmonic generation microscopy reveals the spatial orientation of glutamine-potentiated liver regeneration after hepatectomy
Yen, Chih-Chieh, et al., Hepatology Communications 9.3 (2025): e0640.
Liver fibrosis progression analyzed with AI predicts renal decline
Sun, Dan-Qin, et al., JHEP Reports (2025): 101358.
A machine learning based algorithm accurately stages liver disease by quantification of arteries
Li, Zhengxin, et al., Scientific Reports 15.1 (2025): 3143.
2024
Enhancing Histology Detection in MASH Cirrhosis for Artificial Intelligence Pathology Platform by Expert Pathologist Training
Goodman, Zachary, et al. Liver International Communications, 2024
Utility of AI digital pathology as an aid for pathologists scoring fibrosis in MASH
Abdurrachim, Desiree, et al. Journal of Hepatology (2024).
Digital quantitation of bridging fibrosis and septa reveals changes in natural history and treatment not seen with conventional histology
Naoumov, Nikolai V., et al. Liver international (2024).
Delicate and thin fibrous septa indicate a regression tendency in metabolic dysfunction-associated steatohepatitis patients with advanced fibrosis
Tong, Xiaofei, et al. Hepatology International (2024): 1-15.
Outcome prediction in metabolic dysfunction-associated steatotic liver disease using stain-free digital pathological assessment
Kendall, Timothy J., Chng, Elaine, et al. Liver International (2024).
Second harmonic generation digital pathology with artificial intelligence: breakthroughs in studying fibrosis dynamics and treatment response
Nikolai V Naoumov & Elaine Chng. Future Medicine AI (2024).
AI-based digital pathology provides newer insights into lifestyle intervention-induced fibrosis regression in MASLD: An exploratory study
Yuan, Hai‐Yang, et al. Liver International (2024).
Liver fibrosis showed a two-phase regression rate during long-term anti-HBV therapy by three-time biopsies assessments
Chen, S., Zhou, J., Wu, X. et al. Hepatol Int (2024).
Potential of Using qFibrosis Analysis to Predict Recurrent and Survival Outcome of Patients with Hepatocellular Carcinoma after Hepatic Resection
Hsiao, Chih-Yang, et al. Oncology 1-11 (2024)
Leukotriene B4 receptor 1 (BLT1) does not mediate disease progression in a mouse model of liver fibrosis
Coyne, Erin., et al. Biochem J; 481 (3): 177–190.
JCAD deficiency attenuates activation of hepatic stellate cells and cholestatic fibrosis
Xie, Li, et al. Clinical and Molecular Hepatology 30.2 (2024): 206.
2023
Digital pathology and artificial intelligence in non-alcoholic steatohepatitis: current status and future directions
Ratziu, Vlad, et al. Journal of Hepatology. October 23, 2023
Persistent steatosis correlates with decreased fibrosis regression during anti-HBV treatment in patients with chronic HBV infection
Zhang, Mengyang and Chen, Shuyan, et al. Journal of Medical Virology 95.10 (2023): e29156.
Digital pathology for nonalcoholic steatohepatitis assessment
Arun J. Sanyal, Prakash Jha & David E. Kleiner. Nat Rev Gastroenterol Hepatol 21, 57–69 (2024).
Collagen co-localized with macrovesicular steatosis better differentiates fibrosis progression in non-alcoholic fatty liver disease mouse models
Wang, Xiao-Xiao, et al. Frontiers in Medicine 10 (2023): 1172058.
Caloric Restriction Combined with Immobilization as Translational Model for Sarcopenia Expressing Key- Pathways of Human Pathology
de Jong, Jelle CBC, et al. Aging and Disease 14.3 (2023): 937.
Tropifexor plus cenicriviroc combination versus monotherapy in non-alcoholic steatohepatitis: Results from the Phase 2b TANDEM study
Anstee, Quentin M., et al. Hepatology: 10-1097.
Second-Harmonic Generated Quantifiable Fibrosis Parameters Provide Signatures for Disease Progression and Regression in Nonalcoholic Fatty Liver Disease
Ng, Nicole, et al. Clinical Pathology 16 (2023): 2632010X231162317.
Tropifexor for nonalcoholic steatohepatitis: an adaptive, randomized, placebo-controlled phase 2a/b trial
Sanyal, Arun J., et al. Nature Medicine 29.2 (2023): 392-400.
Machine learning liver histology scores correlate with portal hypertension assessments in nonalcoholic steatohepatitis cirrhosis
Noureddin, Mazen, et al. Alimentary Pharmacology & Therapeutics 57.4 (2023): 409-417.
2022
Digital pathology with artificial intelligence analyses provides greater insights into treatment-induced fibrosis regression in NASH
Naoumov, Nikolai V., et al. Journal of Hepatology 77.5 (2022): 1399-1409.
Developing a New qFIBS Model Assessing Histological Features in Pediatric Patients With Non-alcoholic Steatohepatitis
Liu, Feng, et al. Frontiers in Medicine 9 (2022): 925357.
Artificial Intelligence Improves Pathologist Agreement for Fibrosis Scores in Nonalcoholic Steatohepatitis Patients
Soon, Gwyneth ST, et al. Clinical Gastroenterology and Hepatology (2022).
Breakthroughs in therapies for NASH and remaining challenges
Ratziu, Vlad, et al. Journal of Hepatology 76.6 (2022): 1263-1278.
Multi-photon microscopy for the evaluation of interstitial fibrosis in extended criteria donor kidneys: A proof-of-concept study
So, Wei Zheng, et al. Clinical Transplantation 36.8 (2022): e14717.
Valproic acid modulates collagen architecture in the postoperative conjunctival scar
Seet, Li-Fong, et al. Journal of Molecular Medicine 100.6 (2022): 947-961.
Complexity of ballooned hepatocyte feature recognition: Defining a training atlas for artificial intelligence-based imaging in NAFLD
Brunt, Elizabeth M., et al. Journal of hepatology 76.5 (2022): 1030-1041.
Effects of Valproic Acid and Mitomycin C Combination Therapy in a Rabbit Model of Minimally Invasive Glaucoma Surgery
Seet, Li-Fong, et al. Translational Vision Science & Technology 11.1 (2022): 30-30.
2021
Diet and exercise reduce pre-existing NASH and fibrosis and have additional beneficial effects on the vasculature, adipose tissue and skeletal muscle via organ-crosstalk
van den Hoek, Anita M., et al. Metabolism 124 (2021): 154873.
Predict Early Recurrence of Resectable Hepatocellular Carcinoma Using Multi-Dimensional Artificial Intelligence Analysis of Liver Fibrosis
Liu, I-Ting, et al.Cancers 13.21 (2021): 5323.
Reliable computational quantification of liver fibrosis is compromised by inherent staining variation
Astbury, Stuart, et al. The Journal of Pathology: Clinical Research 7.5 (2021): 471-481.
Assessment of renal fibrosis and anti-fibrotic agents using a novel diagnostic and stain-free second-harmonic generation platform
Bhuiyan, Sadman, et al. The FASEB Journal 35.5 (2021): e21595.
Second Harmonic Generation (SHG) Microscopy and Hepatic Venous Pressure Gradient-based Validation of a Novel Histological Staging System for Alcoholic Hepatitis
Rastogi, Archana, et al. Virchows Archiv 479 (2021): 493-506.
Artificial intelligence in prediction of non-alcoholic fatty liver disease and fibrosis
Wong, Grace Lai‐Hung, et al. Journal of gastroenterology and hepatology 36.3 (2021): 543-550.
Quantification of Extracellular Matrix Features and its Implications in Hepatocellular Carcinoma Patients after Curative Liver Resection
European Association for the Study of the Liver (EASL) Digital Liver Cancer Summit (2021)
2020
Updates in the Quantitative Assesment of Liver Fibrosis for Nonalcoholic Fatty Liver Disease: Histological Perspective
Soon, Gwyneth, and Aileen Wee. Clinical and Molecular Hepatology27.1 (2020): 44.
The Significance of Fibrosis Quantification as a Marker in Assessing Pseudo-Capsule Status and Clear Cell Renal Cell Carcinoma Prognosis
Qin, Caipeng, et al. Diagnostics 10.11 (2020): 895.
Assessment of progressive alterations in collagen organization in the postoperative conjunctiva by multiphoton microscopy
Seet, Li-Fong, et al. Biomedical Optics Express 11.11 (2020): 6495-6515.
Prognostic value of desmoplastic stroma in intrahepatic cholangiocarcinoma
Guedj, Nathalie, et al. Modern Pathology 34.2 (2021): 408-416.
An Improved qFibrosis Algorithm for Precise Screening and Enrollment into Non-Alcoholic Steatohepatitis (NASH) Clinical Trials
Leow, Wei-Qiang, et al. Diagnostics 10.9 (2020): 643.
Multiphoton Microscopic Study of the Renal Cell Carcinoma Pseudocapsule: Implications for Tumour Enucleation
Tan, Yi Quan, et al. Urology 144 (2020): 249-254.
Quantitative stain-free imaging and digital profiling of collagen structure reveal diverse survival of triple negative breast cancer patients
Gole, Laurent, et al. Breast Cancer Research 22 (2020): 1-13.
Long-Term Obeticholic Acid Therapy Improves Histological Endpoints in Patients With Primary Biliary Cholangitis
Bowlus, Christopher L., et al. Clinical Gastroenterology and Hepatology 18.5 (2020): 1170-1178.
Improved quantitative assessment of HBV-associated liver fibrosis using second-harmonic generation microscopy with feature selection
Hsiao, C-Y., et al. Clinics and Research in Hepatology and Gastroenterology 44.1 (2020): 12-20.
Quantitative SHG-microscopy: Unraveling the nano-architecture of the cirrhotic liver
Wanless, Ian R. Clinics and Research in Hepatology and Gastroenterology 44.1 (2019): 1-3.
2008 - 2019
qFIBS: An Automated Technique for Quantitative Evaluation of Fibrosis, Inflammation, Ballooning, and Steatosis in Patients With Nonalcoholic Steatohepatitis
Liu, Feng, et al. Hepatology 71.6 (2020): 1953-1966.
Quantifying and monitoring fibrosis in non-alcoholic fatty liver disease using dual-photon microscopy
Wang, Yan, et al. Gut 69.6 (2020): 1116-1126.
A novel nutritional supplement prevents muscle loss and accelerates muscle mass recovery in caloric-restricted mice
van den Hoek, Anita M., et al. Metabolism 97 (2019): 57-67.
ECM1 Prevents Activation of Transforming Growth Factor β, Hepatic Stellate Cells, and Fibrogenesis in Mice
Fan, Weiguo, et al. Gastroenterology 157.5 (2019): 1352-1367.
SHG/TPEF-based image technology improves liver fibrosis assessment of minimally sized needle biopsies
Wang, Bingqiong, et al. Hepatology International 13 (2019): 501-509.
Digital pathology, what is the future?
Paradis, V., and A. Quaglia. Journal of Hepatology 70.5 (2019): 1016-1018.
Quantification of hepatic steatosis in chronic liver disease using novel automated method of second harmonic generation and two- photon excited fluorescence
Goh, George Boon-Bee, et al. Scientific reports 9.1 (2019): 2975.
Protocols for Culturing and Imaging a Human Ex Vivo Osteochondral Model for Cartilage Biomanufacturing Applications
Duchi, Serena, et al. Materials 12.4 (2019): 640.
qBallooning: Automated Image Detection for Ballooning Degeneration in Non-alcoholic Steatohepatitis (NASH)
Leow, Wei-Qiang, et al. Pathology 51 (2019): S89-S90.
Obeticholic Acid Modulates Serum Metabolites and Gene Signatures Characteristic of Human NASH and Attenuates Inflammation and Fibrosis Progression in Ldlr‐/‐.Leiden Mice
Morrison, Martine C., et al. Hepatology Communications 2.12 (2018): 1513-1532.
Liver Fibrosis Quantification by Digital Whole Slide Imaging and Two Photon Microscopy with Second Harmonic Generation
Jedrzkiewicz, J., et al. Int J Pathol Clin Res 4 (2018): 1-8.
Biofabrication of human articular cartilage: a path towards the development of a clinical treatment
Onofrillo, Carmine, et al. Biofabrication 10.4 (2018): 045006.
Second harmonic generation microscopy provides accurate automated staging of liver fibrosis in patients with non-alcoholic fatty liver disease
Chang, Pik Eu, et al. PLoS One 13.6 (2018): e0199166.
Modeling NAFLD disease burden in China, France, Germany, Italy, Japan, Spain, United Kingdom, and United States for the period 2016-2030
Estes, Chris, et al. Journal of hepatology 69.4 (2018): 896-904.
Advanced septa size quantitation determines the evaluation of histological fibrosis outcome in chronic hepatitis B patients
Wang, Bingqiong, et al. Modern Pathology 31.10 (2018): 1567-1577.
Quantitative Assessment of Liver Fibrosis (qFibrosis) Reveals Precise Outcomes in Ishak “Stable” Patients on Anti-HBV Therapy
Sun, Yameng, et al. Scientific Reports 8.1 (2018): 2989.
INT-767 Improves Histopathological Features in a Diet-induced ob/ob Mouse Model of Biopsy-confirmed Non-alcoholic Steatohepatitis (NASH)
Roth, Jonathan D., et al. World Journal of Gastroenterology 24.2 (2018): 195.
Improved performance of quantitative collagen parameters versus standard histology in longitudinal assessment of nonadvanced liver fibrosis for chronic hepatitis B
Wang, Y., et al. Journal of Viral Hepatitis 25.5 (2018): 598-607.
Second Harmonic Generation Reveals Subtle Fibrosis Differences in Adult and Pediatric Nonalcoholic Fatty Liver Disease
Liu, Feng, et al. American Journal of Clinical Pathology 148.6 (2017): 502-512.
Novel Therapeutic Targets and Emerging Treatments for Fibrosis
Samuel, Chrishan S., and Timothy D. Hewitson. Frontiers in Pharmacology 8 (2017): 824.
Systematic Quantitation of Histologic Patterns Shows Accuracy in Reflecting Cirrhotic Remodeling
Wang, Yan, et al. Journal of Gastroenterology and Hepatology 32.9 (2017): 1631-1639.
Automated Evaluation of Liver Fibrosis in CCI4, BDL and TAA Rodent Models Using Second Harmonic Generation/Two-Photon Excited Fluorescence Microscopy
Liu, Feng, et al. Laboratory Investigation (2016), 1-9.
New classification of liver biopsy assessment for fibrosis in chronic hepatitis B patients before and after treatment
Sun, Yameng, et al. Hepatology 65.5 (2017): 1438-1450.
Quantitative imaging of fibrotic and morphological changes in liver of non-alcoholic steatohepatitis (NASH) model mice by second harmonic generation (SHG) and auto-fluorescence (AF) imaging using two-photon excitation microscopy (TPEM)
Yamamoto, Shin, et al. Biochemistry and Biophysics Reports 8 (2016): 277-283.
Evaluation of Liver Fibrosis: “Something Old, Something New…“
Almpanis, Zannis, et al. Annals of gastroenterology: quarterly publication of the Hellenic Society of Gastroenterology 29.4 (2016): 445.
Automated biphasic morphological assessment of hepatitis B-related liver fibrosis using second harmonic generation microscopy
Wang, Tong-Hong, et al. Scientific reports 5.1 (2015): 12962.
Multiphoton Microscopy: A Potential Intraoperative Tool for the Detection of Carcinoma In Situ in Human Bladder
Jain, Manu, et al. Archives of Pathology and Laboratory Medicine 139.6 (2015): 796-804.
Evaluation of Area-based Collagen Scoring by Nonlinear Microscopy in Chronic Hepatitis C-induced Liver Fibrosis
Sevrain, David, et al. Biomedical optics express 6.4 (2015): 1209-1218.
Low Cost and Compact Nonlinear (SHG/TPE) Laser Scanning Endoscope for Bio-medical Application
Liu, Jia Yun, et al. Endoscopic Microscopy X; and Optical Techniques in Pulmonary Medicine II. Vol. 9304. SPIE, 2015.
Current Strategies for Quantitating Fibrosis in Liver Biopsy
Wang, Yan, et al. Chinese Medical Journal 128.02 (2015): 252-258.
Differentiation of Col I and Col III Isoforms in Stromal Models of Ovarian Cancer by Analysis of Second Harmonic Generation Polarization and Emission Directionality
Tilbury, Karissa, et al. Biophysical journal 106.2 (2014): 354-365.
Minimally Invasive Multiphoton and Harmonic Generation Imaging of Extracellular Matrix Structures in Lung Airway and Related Diseases.
Abraham, Thomas, et al. Pulmonary pharmacology & therapeutics 24.5 (2011): 487-496.
Aligned Collagen is a Prognostic Signature for Survival in Human Breast Carcinoma
Tai, Dean CS, et al. Journal of biomedical optics 14.4 (2009): 044013-044013.
Fibro-C-index: Comprehensive, Morphology-based Quantification of Liver Fibrosis Using Second Harmonic Generation and Two-Photon Microscopy
Tai, Dean CS, et al. Journal of biomedical optics 14.4 (2009): 044013-044013.
Nonlinear Optical Microscopy: Use of Second Harmonic Generation and Two-Photon Microscopy for Automated Quantitative Liver Fibrosis Studies
Sun, Wanxin, et al. Journal of biomedical optics 13.6 (2008): 064010-064010.